studio monitors (fronts) and only review СЃ РхРёР·РєРёРј сопритивЂски сопритим сопрититвтски сопритивм.Induction of heme oxygenase-1 by 5-aminolevulinic acid in human lung epithelial cells.
Previous reports have demonstrated that 5-aminolevulinic acid (ALA), a precursor for heme synthesis in hemopoietic cells, also induces heme oxygenase-1 (HO-1) in these cells. In the present study, we examined whether ALA could induce HO-1 in human lung epithelial cells. ALA (500 microM) enhanced HO-1 mRNA and protein levels in a time-dependent manner. Pretreatment with cycloheximide (100 microg/ml) or actinomycin D (2.5 microg/ml) resulted in the inhibition of ALA-induced HO-1 mRNA and protein. The level of HO-1 mRNA peaked 24 h after ALA stimulation and was inhibited in the presence of protein synthesis inhibitors. Nuclear run-on assay demonstrated that ALA increased the HO-1 gene transcription in a time-dependent manner. Transient transfection of HO-1 promoter-luciferase constructs also demonstrated that ALA-induced HO-1 transcription through the HO-1 promoter in A549 cells. These results demonstrate that ALA could induce HO-1 in human lung epithelial cells. ALA-induced HO-1 expression may be mediated through the increased transcriptional activity of the HO-1 gene.Q:
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